Microchemical Apparatus

Microchemical identification of amidone.

Microchemical analytical techniques of potential clinical interest.

Microchemical techniques with the capability of single-cell analysis will become increasingly important in clinical laboratory practice. One of the current moves underway in this direction is determination of enzyme activities of cells by microscale spectrophotometry and spectrophotofluorometry for prenatal diagnosis of inherited enzyme deficiencies. Three less-common microanalytical techniques...

Microchemical identification of gamma-hydroxybutyrate (GHB)

A new microcrystal test for the detection of gamma-hydroxybutyrate (GHB) is described. The silver/copper reagent consists of an aqueous solution of 0.1 g of cupric nitrate and 0.1 g of silver nitrate in 10.0 mL water. While some crystals form upon evaporation of the reagent, the test forms distinctive crystals for GHB and does not form crystals with some commonly encountered controlled substanc...

Microchemical Deoxyribonucleic Acid Determination in Individual Cells

A method for the quantitative determination of DNA in the 50 to 500 micromicrog. range is presented. Cells or cell nuclei are isolated individually from fixed tissue by means of micromanipulation. The tissue units in question are extracted in an oil chamber with deoxyribonuclease solution. The extracts are evaporated to dryness and redissolved to lens-shaped drops, the DNA contents of which are...

A microchemical determination of desoxyribonucleic acid.